Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...
Propidium iodide staining of DNA in fixed cells has been widely adopted in cell cycle analysis, but it stains all double-stranded nucleic acids, so cells must be treated with RNase before staining ...
Dead cells (propidium iodide-positive) were excluded. Data were evaluated using the Cellquest software.
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