Harvest cells, want good single cell suspension Count cells Dispense aliquots of 1 million cells into labeled tubes Add 5 ml PBS and centrifuge for 5 min at 400 x g. Re-suspend cell pellet in 300 ul ...

Few cell-permeable DNA dyes are excitable at these wavelengths, forcing reliance on time-consuming methods that require cell fixation and permeabilization. Propidium iodide staining of DNA in ...