Precipitate the DNA with ethanol in the presence of 0.3 M sodium acetate (pH 5.2) and dissolve in 90 μl of Tris/EDTA (TE) buffer (pH 7.6). 10| To the DNA, add 10 μl 10× T4 polynucleotide kinase ...

In this manuscript the authors established a novel three-dimensional culture system for stratified epithelia that allows epithelial cells to undergo epithelial-to-mesenchymal transition (EMT) and ...

The enzyme was immobilized using the sodium alginate matrix technique ... measuring activity at 30 ºC over the pH range 4.0 to 9.0, using the following buffers: 50 mM acetate (pH 4.0, 4.5, 5.0, and ...

Cysteine or LAIR1-LP was suspended in an endotoxin-free binding buffer (0.1 M sodium phosphate, 0.15 M sodium chloride ... after being stained with 2% uranyl acetate. NIH/3T3 (ATCC CRL-1658) mouse ...

In this installment, I primarily discuss considerations to keep in mind when choosing buffering additives that will be used ...

However, the study provides limited new insights into the relationship between retinal lamination defects and overall retinal function. Neural lamination is a common feature of the central nervous ...

Let’s get one thing out of the way: Salt and sodium are not the same. Usually when you hear about salt or sodium, the words tend to be tossed around interchangeably, which can be confusing at ...

Sodium is an electrolyte that helps regulate water levels and other bodily substances. Low sodium levels, or hyponatremia, can lead to lethargy, confusion, fatigue, and other symptoms. Treating ...

Sodium-ion batteries show promise as a cheaper, more sustainable alternative to lithium-ion but need major advancements to become competitive. Stanford’s STEER study emphasizes that innovation, not ...