IHC WEBINAR
IHC WEBINAR
May 19, 2021
sigmaaldrich.com
🧬 Immunohistochemistry (IHC) 101 — Part 1 The process in a nutshell IHC helps us identify specific antigens in tissue sections using antibodies. Basic flow: 🔹 Fixation & processing 🔹 Antigen retrieval 🔹 Primary antibody binding 🔹 Detection & visualization 🔹 Interpretation ⚠️ But what if the IHC stain goes wrong? ❌ Weak or no staining ❌ High background / nonspecific staining ❌ False positives or false negatives Most issues trace back to pre-analytical errors, antibody selection, retrieval m
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🧬 IHC 101 – Part 2 | The Theory 🔬 What is IHC? A technique using antibodies to detect antigens in tissue sections. 🧩 Antigen – the target protein 🧷 Antibody – the binder that detects the target 🔍 Monoclonal vs Polyclonal • Monoclonal – high specificity, cleaner staining • Polyclonal – higher sensitivity, more background risk, Understanding these basics is key to accurate IHC results. 🎥 The full ICC video discussion is available exclusively for members. 👉 Learn more at upcoming Philippine
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