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19:17
YouTube
Dr. Jack Auty
How does an ELISpot work?
Learning objectives: Explain what problem the ELISpot solves. Explain how an ELISpot assay works. Describe the purpose of each of these steps. Adhesive plastic plate. Coat the plate in a capture antibody for the cytokine of interest. Block the plate with bovine albumin. Incubate cells with stimuli onto the capture antibodies in the plate. When ...
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