PCR Fundamentals: Components, Thermal Cycling, and Theory
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PCR Fundamentals: Components, Thermal Cycling, and Theory
This video describes the fundamental principles and procedures of the polymerase chain reaction (#PCR), which has become an essential tool for molecular biology since its invention by Kary Mullis in 1983. PCR amplifies a DNA sequence of interest by making many copies of the target sequence, in a process similar to that used by cells during ...
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Rashi Gupta | PhD Scholar on Instagram: "“Polymerase Chain Reaction (PCR) relies on three fundamental steps—denaturation, annealing, and extension🧬—to achieve precise and exponential amplification of a target DNA sequence. During the denaturation phase (94–98°C), the double-stranded DNA unwinds and separates into single strands by breaking hydrogen bonds, preparing the template for amplification. In the annealing step (50–65°C), the temperature is lowered to allow sequence-specific primers to h
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